Ke lilo koke nei ka haloʻo Lion's Mane (Hericium erinaceus) i mea kūʻai aku i ka ʻaha lāʻau lapaʻau ma nā ʻāina he nui ma muli o kāna mau pono neurological a me ka cognitive. ʻOiai e ulu ana kekahi mau hui ma US i kahi ʻano mycelial e like me ka palaoa fermented (mycelial biomass), a ke hoʻonui nei ka helu i ka US a me nā wahi ʻē aʻe e hoʻopuka i kāna mau hua hua no ka hoʻohana ʻana i ka meaʻai, ʻo Kina ka helu ʻekahi o ka mea kanu ʻo Lion's Mane nona ke kuleana ma luna o 90. % o ka hana honua. Aia nā wahi ulu nui ma nā wahi mauna o ka ʻāina ʻo Zhejiang hema a me ka ʻāina ʻo Fujian ʻākau me ka wā e ulu ana mai ʻOkakopa a Malaki.
ʻO ka ʻoihana mahi halo ma Kina, ʻaʻohe kumu kūʻai a ʻaʻole ʻokoʻa ka mahi ʻana o Lion's Mane, ʻoiai hiki ke ulu ʻia ma nā lāʻau lāʻau paʻa holoʻokoʻa, ua ulu maʻamau ia ma nā lāʻau sawdust i hoʻonui ʻia me ka bran palaoa. Eia nō naʻe, ma muli o kona haʻahaʻa haʻahaʻa haʻahaʻa (<0.1%), ʻoi aku ka liʻiliʻi o ka sawdust ma mua o ka substrate maikaʻi loa no ka Lion's Mane e ulu maikaʻi ana ma kahi kiʻekiʻe o ka nitrogen a me kahi haʻahaʻa carbon: nitrogen ratio. No laila, i kēia mau makahiki i hala iho nei, ua hoʻololi nui ka poʻe mahiʻai i ka hui pū ʻana o 90% o nā hulls pulupulu (2.0% nitrogen, 27:1 carbon: nitrogen ratio) a me 8% palaoa bran (2.2% nitrogen, 20:1 carbon: nitrogen ratio) me 1-2% gypsum e kōkua i ka mālama ʻana i ka pH (ʻoi aku ka liʻiliʻi o ka naikokene i loko o nā hull hua pulupulu ma mua o ka bran palaoa akā hoʻopuka i kahi lāʻau me kahi hale ākea ʻoi aku ka maikaʻi no ka ulu ʻana o ka mycelial).
Hāʻawi ʻia nā mea kanu i hoʻohana ʻia no ka hoʻomaʻamaʻa ʻana i kēia mau lāʻau i hana ʻia e nā laboratories e alakaʻi ʻia e ke aupuni panalāʻau a ulu aʻe i mākaukau no ka inoculation e nā hui loea a laila hoʻolako i ka spawn a i ʻole i kekahi mau lāʻau inoculated i ka poʻe mahiʻai. Hoʻopaʻa pū ʻia nā lāʻau inoculated i loko o nā hale ulu i ka wā e noho ana ka mycelium i nā lāʻau i mea e hiki ai i ka wela i hana ʻia e ka mycelium ulu e hoʻolōʻihi i ke kaʻina hana. Ke hoʻopaʻa piha ʻia ma hope o 50-60 mau lā, hoʻoneʻe ʻia nā plugs mai nā wahi inoculation, e hoʻolauna ana i kahi gradient moisture a hoʻomaka i ka hoʻokumu ʻana o nā kino hua. A laila kau ʻia nā lāʻau ma luna o nā pā lāʻau.
ʻO ka Lion's Mane ka maʻalahi i nā loli o ka mahana a me nā kūlana lewa. ʻO ka mahana maikaʻi loa no ka ulu ʻana o ka mycelial ma kahi o 25°C a ʻo ka hoʻokumu ʻana o ke kino hua mai ka 14-25°C me ka 16-18°C maikaʻi loa (ma nā haʻahaʻa haʻahaʻa ua ʻulaʻula nā kino hua a ma nā mahana kiʻekiʻe e ulu wikiwiki lākou akā ʻoi aku ka melemele a me ka liʻiliʻi. me nā spines lōʻihi). Hoʻopili pū nā kino hua i nā pae CO2, e hoʻomohala ana i kahi ʻano coralliform ke piʻi ka pae ma luna o 0.1% (pono i ka ea kūpono) a me ka māmā, e ulu maikaʻi ana i nā kūlana malu.
Mai ka wehe ʻana i nā palaka a hiki i ka puka ʻana o nā kino hua ʻai ma kahi o hoʻokahi pule e pili ana i ka mahana o ka ambient a i kēia manawa e hoʻohuli pinepine ʻia nā lāʻau me ka manaʻoʻiʻo ma ka ulu ʻana i luna-lalo e ʻoi aku ka maikaʻi o nā kino hua a kiʻi i kahi. kumukūʻai kiʻekiʻe.
Ma hope o 7-12 mau lā, mākaukau nā kino hua e ʻohi. Hana ʻia ka ʻohi ʻana ma mua o ka hoʻomaka ʻana e hoʻomohala i nā protuberances elongated e hāʻawi ai i ka halo i kona inoa akā e paʻakikī ai ka lawelawe ʻana i ka halo maloʻo a hele pū ʻia me kahi hale ākea ʻaʻole kūpono no ka hoʻohana ʻana.
Ma hope o ka ʻohi ʻia ʻana, hoʻomaʻemaʻe ʻia nā kino hua mai nā mea ʻē aʻe a maloʻo, i ka lā inā kūpono ke ʻano a i ʻole i loko o nā umu maloʻo i hoʻoulu ʻia e nā lāʻau i hoʻopau ʻia (ma hope o ka wehe ʻana i ko lākou mau lima lima i hoʻouna ʻia no ka hana hou ʻana). E helu ʻia nā kino hua maloʻo e like me ka nui a me ke ʻano me nā mea maikaʻi aʻe i kūʻai ʻia no ka hoʻohana ʻana i ka meaʻai a ʻo nā mea liʻiliʻi liʻiliʻi e wili ʻia i loko o ka pauka a i ʻole ka hana ʻia i ʻano ʻala.
Me kekahi o nā pūhui ʻoi loa o ka neurologically mai ka Lion's Mane e like me ka erinacine A i hoʻokaʻawale ʻia mai ka mycelium ma mua o ke kino huaʻai ke hoʻonui nei ka hana ʻana o ka Lion's Mane mycelium ma Kina. ʻAʻole like me ka paʻa-state fermentation maʻamau ma USA, ma Kina ua mahi ʻia ka mycelium ma luna o ka substrate wai e hiki ke hoʻokaʻawale ʻia mai ka mycelium ma ka hopena o ka fermentation.
Ma kēia ʻano, ua hoʻomākaukau ʻia ka moʻomeheu hoʻomaka ma ke ʻano maʻamau a laila mahi ʻia i loko o kahi pahu reactor pani ma luna o kahi substrate wai i loaʻa ka pauka hū a me ka palaoa kulina a i ʻole ka palaoa soy me ka 3% glucose a me ka 0.5% peptone. ʻO ka nui o ka manawa hana he 60 mau lā a ʻoi aʻe paha me ka hopena o ka fermentation i hoʻoholo ʻia e like me ke kō i loko o ka wai fermentation.
I like me nā halo ʻē aʻe a me ka ʻaelike me kona hoʻohana ʻana i ka Traditional Chinese Medicine (TCM) Lion's Mane extracts ua hana nui ʻia e ka ʻili wai wela. Eia nō naʻe, me ka hoʻonui nui ʻana i kāna mau pono neurological a me ka ʻike ʻana o nā pūhui nui i ʻike ʻia e hāʻawi ana i kāna hana ma kēia wahi ua maʻalahi ka hoʻoheheʻe ʻana i nā solvents e like me ka waiʻona i kēia manawa ua piʻi ka hoʻonui ʻana i ka lawe ʻana i ka waiʻona, me ka ʻaila ʻona i kekahi manawa. hui pū ʻia me ka ʻāpana wai ma ke ʻano he 'lua-extract'. Lawe ʻia ka ʻili wai ma ka hoʻolapalapa ʻana no 90 mau minuke a laila kānana e hoʻokaʻawale i ka ʻili wai. I kekahi manawa, hana ʻia kēia kaʻina hana ʻelua me ka hoʻohana ʻana i ka pūʻulu like o ka halo maloʻo, ʻo ka lua o ka unuhi ʻana e hāʻawi i kahi hoʻonui liʻiliʻi o ka hua. A laila hoʻohana ʻia ka manaʻo hoʻomāmā (hoʻomaʻamaʻa a hiki i 65°C ma lalo o kahi ʻāpana ʻāpana) no ka wehe ʻana i ka hapa nui o ka wai ma mua o ka hoʻohu ʻana-maloʻo.
E like me ka Lion's Mane aqueous extract, i like me nā extracts o nā halo ʻai ʻē aʻe e like me Shiitake, Maitake, Oyster Mushroom, Cordyceps militaris a me Agaricus subrufescens ʻaʻole i loaʻa wale nā polysaccharides kaulahao lōʻihi akā he mau kiʻekiʻe kiʻekiʻe o nā monosaccharides liʻiliʻi, disaccharides a me nā oligosaccharides ʻaʻole hiki ke hoʻopuʻi - maloʻo e like me ke ʻano a i ʻole nā wela kiʻekiʻe i loko o ka hale kiaʻi e hoʻomaloʻo i ka nā kō liʻiliʻi e caramelise i loko o kahi puʻupuʻu paʻa e pale ai i ka puka ʻana mai ka hale kiaʻi.
No ka pale ʻana i kēia maltodextrin (25-50%) a i ʻole i kekahi manawa e hoʻohui pinepine ʻia ke kino hua pauda ma mua o ka hoʻomaloʻo ʻana. ʻO nā koho ʻē aʻe, ʻo ia ka umu-hoʻomaloʻo a me ka wili ʻana a i ʻole ka hoʻohui ʻana i ka waiʻona i ka ʻāpana wai e hoʻoheheʻe i nā molekala nui a laila hiki ke kānana ʻia a maloʻo ʻoiai e noho ana nā molekole liʻiliʻi i loko o ka supernatant a hoʻolei ʻia. Ma ka hoʻololi ʻana i ka ʻona waiʻona hiki ke hoʻomalu ʻia ka nui o nā molekala polysaccharide i hoʻopaʻa ʻia a hiki ke hana hou ʻia ke kaʻina hana inā pono. Eia naʻe, ʻo ka hoʻolei ʻana i kekahi o nā polysaccharides ma kēia ala e hōʻemi ai i ka hua a no laila e hoʻonui ai i ke kumukūʻai.
ʻO kahi koho ʻē aʻe i noiʻi ʻia ma ke ʻano he koho no ka wehe ʻana i nā moleke liʻiliʻi ʻo ia ka kānana membrane akā ʻo ke kumukūʻai o nā membranes a me ko lākou ola pōkole ma muli o ke ʻano o nā pores e hoʻopaʻa ʻia e hiki ʻole ke hoʻokele waiwai i kēia manawa.
E like me ka mea i ʻōlelo ʻia ma luna nei, ʻaʻole ʻo ka wai wale nō ka mea hoʻoheheʻe e hiki ke hoʻohana ʻia e unuhi i nā pūhui ikaika mai ka Lion's Mane me ka waiʻona-extraction e lilo i mea maʻamau ma muli o kona hiki ke kiʻi i nā pūhui e like me nā hericenones a me nā erinacines e pili ana i ka hoʻoikaika ʻana i ke kumu ulu aʻalolo ( NGF) hanauna. I kēia hihia, hoʻohana ʻia ia ma kahi ʻano 70-75% me ka hoʻoneʻe ʻia ʻana o ka waiʻona no ka hana hou ʻana ma mua o ka hoʻopuʻi ʻana - maloʻo.
Aia ma kahi o 4:1 ka lākiō kaona o ka wai maloʻo ma kahi o 4:1 ʻoiai hiki ke piʻi i ka 6:1 a i ʻole 8:1 ma hope o ka waiʻona-ua ua ʻoiai ʻo ka neʻe ʻana o ka waiʻona maloʻo ma kahi o 20:1 (a i ʻole 14:1 inā hoʻohana ʻia ka mycelium i hana ʻia. ma ka fermentation wai).
Me ka hoʻonui nui ʻana o ka hoihoi i nā pono olakino o Lion's Mane, ua hoʻonui ʻia ka nui o nā huahana i loko o nā ʻano like ʻole. E like me ka wai a me ka ethanolic, ua hui pū ʻia nā helu ʻelua ma ke ʻano he ʻelua-extract aʻo nā mea ʻē aʻe he nui ka wai i hoʻomaloʻo pū ʻia me ka fiber mushroom hiki ʻole ke hoʻoheheʻe ʻia e like me ka pauka maloʻo a i ʻole 1:1 extract. Me ka Lion's Mane e ʻike ʻia ana i loko o nā meaʻai hana e like me nā kuki, e hauʻoli ke ʻike i nā mea e hiki mai ana no kēia ʻano ʻala.
Ka manawa kau:Iula-21-2022