Cordycepin, cyangwa 3 ′ - deoxyadenosine, ni inkomoko ya nucleoside adenosine. Nibintu bioaktike ishobora gukurwa mubwoko butandukanye bwibihumyo bya Cordyceps, harimo Cordyceps militaris na Hirsutella sinensis (fermentation mycelium ya ophiocordyceps sinensis).
Hagomba kwerekanwa byumwihariko ni uko ikizamini cyerekana umubiri wera imbuto ya ophiocordyceps sinensis isanzwe idafite cordycepin, ariko ifite ibintu byinshi birimo ibyuma biremereye, cyane cyane Arsenic.
Inzira yo gukuramo cordycepin irashobora gukorwa binyuze mu ntambwe zikurikira:
1. Guhitamo amoko y'ibihumyo: Intambwe yambere ni uguhitamo ubwoko bukwiye bwibihumyo bya Cordyceps kugirango bikurwe. Cordyceps militaris ikunze gukundwa kuko irimo urwego rwinshi rwa cordycepine kurusha ubundi bwoko. Hirsutella ihenze cyane kugirango ikurwe. Cordyceps militaris niyo mahitamo yambere kugeza ubu.
2. Guhinga ibihumyo: militaris ya cordyceps ihingwa ahantu hagenzuwe kugirango habeho gukura neza no gutanga umusaruro wa cordycepin. Ibi bishobora kuba bikubiyemo gukura ibihumyo kuri substrate, nk'umuceri cyangwa soya, mubihe by'ubushyuhe, ubushuhe, n'umucyo.
Mubisanzwe duhitamo ibice byambere bya cordycepin murwego rwa 0.1 - 0.3% (ikenera insimburangingo, umuceri nifu ya soya). Mubisanzwe, cordyceps militaris na substrate ya bran ingano ifite 0.05% cordycepin cyangwa irenga.
3.Gusarura no gukama: Agahumyo kamaze gukura, karasarurwa kandi kakuma kugirango gakureho ubuhehere bukabije.
4. Gukuramo cordycepine: Ibikoresho byumye byumye hanyuma bigashyirwa mu ifu nziza hanyuma bigakurwa hakoreshejwe umusemburo ukwiye. Cordycepin irashobora gushonga haba mumazi ndetse nigisubizo cya Ethanol.Musanzwe dukoresha kuvoma amazi kuko byerekana imikorere myiza kandi byoroshye kugenzura.
Ingingo yo gukuramo amazi kugirango ibone cordycepin ni ukugenzura ubushyuhe munsi yagaciro, mubisanzwe munsi ya dogere 70. Bitabaye ibyo, bizaba hydrolyzed byoroshye.
5.Gusukura: Ibikomoka kuri peteroli bivamo noneho bisukurwa hakoreshejwe uburyo butandukanye nka chromatografiya, imvura, cyangwa kristalisiti kugirango bitandukane na cordycepin.
Mubikoresho byacu, dukoresha chromatografiya (cationic resin) kugirango dukore ibintu byinshi bya cordycepin kuva 5% kugeza 95% (uyu numubare ntarengwa twakoze kugeza ubu)
Mubisanzwe, cordycepin kuva 0.5% - 3% ntabwo isabwa kwezwa.
6.Gusesengura no kugerageza: Igicuruzwa cyanyuma kirasesengurwa kandi kigeragezwa kubwera, imbaraga, nubwiza kugirango byuzuze ibyifuzo byifuzwa.
Muri rusange rero, uburyo bwo kuvoma burimo: Gukuramo amazi, Kwiyungurura, Kwibanda, Kweza, Kuma, Gukata, no kumenya ibyuma.
Igeragezwa rya cordycepin ryashizweho neza. Muri make, kugirango ukoreshe HPLC hamwe nurugero rwa Cordycepin. Inkingi zisanzwe zikoreshwa mugutandukanya cordycepin ni C18 inkingi zifite ubunini bwa 3 - 5 µm n'uburebure bwa mm 150 - 250 mm. Tera imeri niba ushaka kumenya byinshi kubyerekeye.
Ikindi kintu kimwe, substrate nyuma yumubiri wera wa cordyceps militaris isarurwa nayo irimo umubare muto wa cordycepin. Ibikuramo rero birashobora kugira 0.2 - 0.5% cordycepin.
Igihe cyo kohereza: Gicurasi - 16 - 2023