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Cordyceps militaris yakasarudzika uye yakakosha yekurapa fungus muChinese Cordyceps, iyo yakashandiswa zvakanyanya sebiocontrol vamiririri muChina kwemazana emakore.

Cordycepin yakapatsanurwa zvakabudirira kubva kuCordyceps mauto ichishandisa kudhirowa nemvura chete pasi peimwe tembiricha, kana musanganiswa we ethanol nemvura. Optimum tembiricha, mvura kana kuumbwa kwe ethanol mumvura, solvent/solid ratio uye pH ye solvent zvakatemwa maererano negoho rekuchera. Mukoho wepamusoro we cordycepin (90% +) wakafanotaurwa nemuenzaniso wekudzorera uye wakasimbiswa nekuenzanisa nemigumisiro yekuedza, kuratidza kubvumirana kwakanaka. Iyo RP-HPLC nzira yakashandiswa kuongorora cordycepin kubva kuCordyceps militaris zvinyorwa, uye 100% kuchena kwecordycepin kwakawanikwa. Maitiro ekubvisa akaongororwa maererano nekuenzanisa uye kinetics.

Mamwe matipi nezve musiyano uripo pakati peCS-4 neCordyceps sinensis neCordyceps militaris

1. CS-4 inomirira kuti cordyceps sinensis nhamba 4 fungal strain —-Paecilomyces hepiali - iyi ifungi endoparasitic inowanzova mu natural cordyceps sinensis.

2. Paecilomyces hepiali yakanga yakaparadzaniswa kubva kune yakasikwa cordyceps sinensis, uye inoculated pane yakagadzirwa substrates (yakasimba kana mvura) kuti ikure. Iyi inzira yekuvirisa. solid substrate —solid status fermentation (SSF), liquid substrate—Submerged fermentation (SMF).

3. Kusvika pari zvino chete cordyceps militaris (iyi nderimwe rudzi rwe cordyceps) 's mycelium uye fruiting body has cordycepin.  uye kune rumwe rudzi rwe cordyceps ( Hirsutella sinensis ) , inewo cordycepin . Asi Hirsutella sinensis inongova mycelium inowanikwa.