ICordycepin, okanye 3′-deoxyadenosine, iphuma kwinucleoside adenosine. Yikhompawundi yebioactive enokukhutshwa kwiindidi ezahlukeneyo zomngundo weCordyceps, kuquka iCordyceps militaris kunye neHirsutella sinensis (i-artificial fermentation mycelium ye ophiocordyceps sinensis).
Kufuneka kucaciswe ngokukodwa ukuba uvavanyo lubonisa umzimba weziqhamo we-natual ophiocordyceps sinensis ayinayo i-cordycepin, kodwa inomxholo ophezulu weentsimbi ezinzima, ngakumbi i-Arsenic.
Inkqubo yokutsalwa kwe-cordycepin inokwenziwa ngala manyathelo alandelayo:
1. Ukukhethwa kweentlobo zokungunda: Inyathelo lokuqala kukukhetha iintlobo ezifanelekileyo ze-Cordyceps fungus ukuze zikhutshwe. ICordyceps militaris ihlala ikhethwa kuba inamanqanaba aphezulu e-cordycepin kunezinye iintlobo. I-Hirutella ibiza kakhulu ukwenza utsalo. Ke i-cordyceps militaris lukhetho lokuqala ukuza kuthi ga ngoku.
2.Ukulinywa kwefungus: I-cordyceps militaris ikhuliswa kwindawo elawulwayo ukuze kuqinisekiswe ukukhula okulungileyo kunye nokuveliswa kwe-cordycepin. Oku kunokubandakanya ukukhulisa umngundo kwisubstrate, efana nerayisi okanye imbotyi yesoya, phantsi kweemeko ezithile zobushushu, ukufuma kunye nokukhanya.
Ngokuqhelekileyo sikhetha umxholo wokuqala we-cordycepin kuluhlu lwe - 0.1-0.3% ( lufuna i substrates ezithile , irayisi kunye nomgubo wesoya ) . Ngokuqhelekileyo, i-cordyceps militaris yi-substrates yengqolowa yengqolowa ine-0.05% ye-cordycepin okanye nangaphantsi.
3. Ukuvuna nokomisa: Xa umngundo ufikelele ekuvuthweni, uyavunwa kwaye womiswe ukususa ukufuma okugqithisileyo.
4. Ukutsalwa kwe-cordycepin: Izinto zokungunda ezomisiweyo zigatywa zibe ngumgubo ocolekileyo kwaye zixhomekeke ekutsalweni kusetyenziswa i-solvent efanelekileyo. I-Cordycepin inokunyibilika kumanzi kunye nesisombululo se-ethanol. Ngokuqhelekileyo sisebenzisa ukutsalwa kwamanzi kuba kubonisa ukusebenza kweendleko ezingcono kwaye kulula ukulawula.
Inqaku lokukhutshwa kwamanzi ukufumana i-cordycepin kukulawula ubushushu phantsi kwexabiso elichaziweyo, ngokuqhelekileyo phantsi kwe-70 degree centigrade. Kungenjalo, iya kwenziwa i-hydrolyzed ngokulula.
I-5.I-Purification: I-extract crude ephumayo ihlanjululwa ngokusebenzisa iindlela ezahlukeneyo ezifana ne-chromatography, imvula, okanye i-crystallization ukuhlukanisa i-cordycepin compound.
Kwindawo yethu, sisebenzisa i-chromatography(i-cationic resin) ukwenza umxholo ophezulu we-cordycepin ukusuka kwi-5% ukuya kwi-95% (eli lelona nani liphezulu esilenzileyo ukuza kuthi ga ngoku)
Ngokuqhelekileyo, i-cordycepin esuka kwi-0.5%-3% ayifuni ukuba ihlanjululwe.
I-6.Uhlalutyo kunye novavanyo: Imveliso yokugqibela ihlalutywa kwaye ivavanywe ukucoceka, amandla, kunye nomgangatho wokuqinisekisa ukuba ihlangabezana neenkcukacha ezifunwayo.
Ke iyonke, inkqubo yokutsalwa ibandakanya: Ukutsalwa kwamanzi, ukuhluzwa, ukugxila, ukucocwa, ukomisa, ukuhluza, kunye nokufunyanwa kwesinyithi.
Uvavanyo lwe-cordycepin lusekwe kakuhle. Ngokufutshane, ukusebenzisa i-HPLC kunye nesampulu yereferensi yeCordycepin. Iikholamu eziqhelekileyo ezisetyenziselwa ukwahlula i-cordycepin ziintsika ze-C18 ezinobungakanani besuntswana le-3-5 µm kunye nobude be-150-250 mm. Sithumele i-imeyile ukuba ufuna ukwazi ngakumbi ngayo.
Enye into, i-substrates emva kokuba umzimba we-cordyceps militaris uvuniwe ukwanalo inani elincinci le-cordycepin. Ngoko isicatshulwa sayo sinokuba ne-0.2 - 0.5% ye-cordycepin.
Ixesha lokuposa:May-16-2023