I-Cordycepin, noma i-3 '- I-Deoxyadenosine, iyona etholwa yi-adenosine ye-nucleoside. Yikhompiyutha ebingasebenzi engakhishwa ezinhlotsheni ezahlukahlukene ze-CorderyPs fungus, kufaka phakathi i-CordeySyPS Militaris ne-Hirsutella Sinetnsis (i-mycelium yokufakelwa yokufakelwa kwe-mycelium ye-opiocooryyys ye-opihoocooryys.
Kudinga ukuboniswa ikakhulukazi ukuthi isivivinyo sibonisa umzimba wezithelo wemvelo we-ophiocooryyseps i-soniocooryces ayinayo i-cordcensin, kepha inokuqukethwe okuphezulu kwezinsimbi ezisindayo, ikakhulukazi i-arsenic.
Inqubo yokukhishwa kweCordicyPin ingenziwa ngezinyathelo ezilandelayo:
1. Ukukhetha kwezinhlobo ze-fungal: Isinyathelo sokuqala ukukhetha izinhlobo ezifanele ze-CorderyPs fungus yokukhishwa. I-Cordenceps Militaris ivame ukuthandwa ngoba inamazinga aphezulu weCordiryPin kunezinye izinhlobo zezilwane. IHirsupella ibiza kakhulu ukwenza ukukhishwa. I-Cordenceps Militaris ukukhetha kokuqala kuze kube manje.
I-2.Cultivation of funtus: IMilitaris ye-Cordences itshalwa endaweni elawulwayo ukuqinisekisa ukukhula okuphezulu nokukhiqizwa kweCardcePin. Lokhu kungafaka ukukhulisa ukhunta engxenyeni engezansi, efana nelayisi noma i-soya, ngaphansi kwezimo ezithile zokushisa, umswakama, nokukhanya.
Imvamisa sikhetha okuqukethwe kokuqala kwe-CordiryPin ebangeni elingu-0,1 - 0.3% (lidinga ama-substrates athile, irayisi ne-soybean powder). Imvamisa, ama-militaris e-milestrates kakolweni we-bran kuphela ane-0,05% contrycepin noma ngaphansi.
3.Revesting and yomile: Uma nje ukhunta selufinyelele ekuvuthweni, kuvunwa futhi komiswe ukususa umswakama owengeziwe.
I-4. I-Extraction ye-CordencePin: Izinto ezenziwe ngefunguna zibe bese zingena emkhakheni omuhle futhi zikhishwe kusetshenziswa i-solvent efanelekile. I-CordiryPin ingancibilika kuwo womabili la manzi nase-ethanol solution.Sisebenzise ngokuvamile ukukhishwa kwamanzi ngoba kukhombisa ukusebenza kwezindleko ezingcono futhi kulula ukulawula.
Iphuzu lokukhishwa kwamanzi ukuze lithole ama-cordcepin ukulawula amazinga okushisa ngaphansi kwenani elithile, ngokujwayelekile ngaphansi kwama-centigrade angama-70. Ngaphandle kwalokho, kuzobe ku-hydrolyzed kalula.
5.Puratification: I-crude ekhishwe yi-crude yahlanzwa kusetshenziswa izindlela ezahlukahlukene ezinjenge-chromatography, i-teapitation, noma i-crystallization ukuhlukanisa i-contrycepin Compound.
Esikhungweni sethu, sisebenzisa i-chromatography (i-cationic resin) ukwenza okuqukethwe okuphezulu kwe-CordencePin kusuka ku-5% kuya ku-95% (leli inani eliphakeme esilwenzile kuze kube manje)
Ngokuvamile, i-cordcepin kusuka ku-0.5% - ama-3% akudingeki ahlanzeke.
I-6.Analysis kanye nokuhlola: Umkhiqizo wokugcina uyahlaziywa futhi uhlolwe ubumsulwa, i-potency, nekhwalithi yokuqinisekisa ukuthi ihlangabezana nokucaciswa okufunayo.
Ngakho-ke, inqubo yokukhishwa ifaka phakathi: Ukukhishwa kwamanzi, ukufomatha, ukuwohloka, ukuhlanzwa, ukomisa, ukufihla, nokutholwa kwensimbi.
Ukuhlolwa kwe-CordingcePin kusungulwe kahle. Kafushane nje, ukusebenzisa i-HPLC ngesampula yereferensi ye-CordiryPin. Amakholomu ajwayelekile asetshenziselwa ukwahlukanisa kweCordncePin amakholomu we-C18 anosayizi we-3 - 5 μm nobude obungu-150 - 250 mm. Silahle i-imeyili uma ufuna ukwazi kabanzi ngayo.
Enye into, ama-substrate ngemuva komzimba wezithelo weMilitaris avunwa futhi aqukethe inani elincane le-CordiryPin. Ngakho-ke ukukhishwa kwalo kungaba ngu-0,2 - 0.5% we-cordrycepin.
Isikhathi Seposi: Meyi - 16 - 2023